Fig 1: MiR-636 overexpression reversed TGF-β1-induced tenogenic differentiation in TDSCs by MAPK1. A Western blotting was adopted to verify the transfection efficiency of pcDNA or MAPK1. B–F MiR-636 alone or miR-636 and MAPK1 were transfected into TDSCs, and then subjected to TGF-β1 treatment. B Western blotting analysis for the levels of SCX, MKX, COL1A1 and FMOD. C, D Cell proliferation detection by CCK-8 and EdU assays (scale bar, 50 μm). E Transwell assay for cell invasion analysis (scale bar, 50 μm). F Wound healing assay for cell migration analysis. **P < 0.01, ***P < 0.001, ****P < 0.001
Fig 2: Circ_0005736 silencing reversed TGF-β1-induced tenogenic differentiation in TDSCs. A The knockdown efficiency of si-NC and si-circ_0005736 was validated using qRT-PCR. B–G TDSCs were transfected with si-circ_0005736 or si-NC, followed by TGF-β1 treatment. B Western blotting analysis for the levels of SCX, MKX, COL1A1 and FMOD. C, D Cell proliferation detection by CCK-8 and EdU assays (scale bar, 50 μm). E, F Transwell assay for cell invasion analysis (scale bar, 50 μm). G Wound healing assay for cell migration analysis. **P < 0.01, ***P < 0.001, ****P < 0.001
Supplier Page from Abcam for Anti-Fibromodulin antibody [EPR23029-174]